Biological Research Department

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Provides non-animal pre-clinical research and testing for evaluating toxicity and efficacy. Biological research department has developed various in-vitro methods for claim substantiation of products to determine the efficacy and safety of products by pre-clinical assays.

The team has experience in in-vitro assay studies conducted worldwide, as well as in serving the testing needs of diverse industrial clients. Our familiarity with an array of different assay systems and product lines gives us the knowledge and expertise to determine the optimum assay for specific test materials and applications. Biological research lab is NABL accredited and experienced in performing in-vitro procedures in compliance with Good Laboratory Practice (GLP).

Safety Studies

  • Dermal Irritection® Assay System 
  • Ocular Irritection®  Assay System 
  • Cytotoxicity Studies
  • Bacterial Reverse Mutational Assay
  • Others
    • Gluten Assay 
    • Cholestrol Ester Transfer Protein (CETP) Inhibitory Assay

Efficacy Models

  1. Immunomodulatory Studies
    • Innate
      • Estimation of Cytokines from Activated Neutrophils, Macrophages, Monocytes.
    • Adaptive
      • Immunophenotyping
        • Expression of Specific Cell Surface Activation Antigens... CD3, CD4, CD8, CD19, CD21, CD25,CD45, CD80, CD86 etc.
        • TH1 (IL-2, IFN-gamma, TNF-alpha)
        • Th2(IL-4, IL-10, IL-13 etc.)
  2. Anti Inflammatory
    • Estimation of Pro-inflammatory and Anti-inflammatory Cytokines, Chemokines and Mediators.
    • Estimation of Complement Proteins.
    • Estimation of Interleukins.
    • Estimation of Growth Factors and Adhesion Molecules.
    • Estimation of Degrading Enzymes.
    • Inhibition of Nitric Oxide- iNOS
    • Estimation of Prostaglandins- PGE2
  3. Anti Oxidant
    • DPPH Radical Scavenging Activity
    • Oxygen Radicals Absorbance Capacity (ORAC)
    • Hydroxyl Radicals Antioxidant Capacity (HORAC)
    • Nitroxide Radicals Antioxidant Capacity (NORAC)
    • Inhibition of Lipid Peroxidation
    • Cell Based Inhibition of Reactive Oxygen Species (ROS)
  4. Anti Obesity
    • Anti Lipase Assay
    • Inhibition of Leptin, Adiponectin and Adipokines
    • Cell Based Assay – Inhibition of Adipogenesis
  5. Anti Diabetes
    • Alpha Glucosidase Inhibitory Assay
    • DPP IV Inhibitory Assay
    • 11 β- Hydroxy Steroid Dehydrogenase type I (11 β HSD I) Inhibitory Assay 
    • Neutral Endopeptidase (NEP) Inhibitory Assay
    • Protein Tyrosinase Phosphatase (PTP IB) Inhibitory Assay
    • Alpha Amylase Inhibition Activity
    • Aldose Reductase Inhibition assay
    • Glycation
  6. Anti Microbial
    • S.aureus
    • S. epidermidis
    • E. coli
    • C.albicans
    • M.furfur
    • P.acne
  7. Anti Cancer
    • Cyto-Toxicity Assay
    • Apoptosis Assays
    • Anti Inflammatory Activity
    • Antioxidant Activity Studies
    • Immunomodulation Assay
    • General Pharmacological Studies
  8. Hair Care
    • Cell Proliferation
    • 5-Alpha Reductase Inhibition
  9. Skin lightening/Tanning
    • Melanin Synthesis Inhibition
    • Melanin Production Acceleration
    • Inhibition of Tyrosinase
    • UV Protection Assay using Cell Lines
  10. Anti Ageing
    • Anti Elastase Assay
    • Anti Collagenase Assay
    • Anti Hyaluronidase Assay
    • Cell Based Assay for Collagen Enhancement
    • Antioxidant Studies
    • Transforming Growth Factor Beta (TGF-β) Enhancement
    • Degradation of Matrix Metalloproteinases (MMPs)
    • Enhancement of FGF

List of Main Equipments

  • FACS Canto II Flowcytometer
  • Real Time PCR
  • UV- Visible Spectrophotometer
  • Plate Reader
  • CO2 Incubator
  • BOD Incubator
  • Fluostar Optima
  • Biological Safety Cabinets
  • ULT Freezer
  • Cooling Centrifuge
  • Tecan Sunrise Plate Reader